November 18-24, 2012

Ljubljana, Slovenia

Electroporation based Technologies and Treatments



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and try out DEMO of EBTT distant learning!

Practical work:

You will be assigned to practical work sessions only after you have paid the registration fee and have passed the laboratory safety test. Your chosen practical work preferences will be taken into account, until the places for practical work sessions are filled. Sessions with a limited number of participants will be filled on a first-come-first-served basis. You can select up to three practical work sessions from wet lab and modelling and both from computer simulation and e-learning. Please be sure to mark all practical work sessions by priority, as both wet and modelling sessions have limited number of participants, therefore it's possible that your second or third choice will be considered instead of your first one.

Laboratory safety test:

S1 - Laboratory safety

Please Sign-in/Login to the education system (in the left navigation menu). Use equal e-mail address that you used it on registration form. In the education system, you will find Laboratory safety test course. On the left side of the menu click test. Solve the questionary and submit it.

List of exercises:

L1 - The influence of Mg2+ ions on gene electotransfer efficiency

L2 - Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM

L3 - Electropermeabilization detection with propidium iodide in plated cells

L4 - Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS

Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS on JOVE

L5 - Irreversible electroporation of bacterial cells

L6 - Electroporation of planar lipid bilayers

L7 - Cell electrofusion

Cell electrofusion on JOVE

L8 - Analysis of electric field orentations on gene electrotransfer - efficiency and visualization at the membrane level

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro on JOVE

L9 - Skin electroporation

L10 - PEF-induced crytoprotection of spinach leaves

C1 - Electrochemotherapy treatment planning: optimization of voltage and electrode position

C2 - Numerical modeling of coupled physical phenomena

C3 - Molecular dynamics simulations of membrane electroporation

E1 - Electroporation of cells and tissues - interactive e-learning