November 18-24, 2012

Ljubljana, Slovenia

Electroporation based Technologies and Treatments



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and try out DEMO of EBTT distant learning!

Key lectures and content of the course:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
  • Dielectric properties of biological tissues
  • Temperature and conductivity changes in tissues
  • Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
  • Molecular dynamics simulations
  • Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
  • Electroporation of cells in vivo
  • Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
  • Electrodes for in vitro and in vivo
  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
  • Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
  • Preclinical studies
  • Clinical use of electrochemotherapy
  • Current developments and future perspectives
  • Application of electroporation in gene transfection

  • Gene transfection in vitro
  • Gene transfection in vivo
  • Clinical use in human medicine
  • Electroporation in transdermal drug delivery

  • Electroporation for gene delivery in the skin
  • Electroporation for DNA vaccine
  • Additional topics:

  • Molecular dynamics simulations of lipid bilayers electroporation, Mounir Tarek
  • VNon-thermal irreversible electroporation as a tissue ablation therapy, Rafael Davalos
  • Cell-specific targeting strategies for electroporation-mediated gene delivery in the lung, David A. Dean
  • Safety and toxicity of electroporation, Julie Gehl
  • Electroporation in food processing, Stefan Töpfel
  • Laboratory and practical work

  • Gene electrotransfer with different pulse parameters
  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
  • Electropermeabilization detection of attached cells with propidium iodide
  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
  • Electroporation of bacteria
  • Electroporation of planar lipid bilayers
  • Cell electrofusion
  • Electrochemotherapy treatment planning: optimization of voltage and electrode position
  • Numerical modeling of coupled physical phenomena
  • Molecular dynamics simulations of membrane electroporation
  • Electrochemotherapy - interactive learning